A simple and fast Agrobacterium-mediated transformation system for passion fruit KPF4 (Passiflora edulis f. edulis × Passiflora edulis f. flavicarpa).
Identifieur interne : 000058 ( Main/Exploration ); précédent : 000057; suivant : 000059A simple and fast Agrobacterium-mediated transformation system for passion fruit KPF4 (Passiflora edulis f. edulis × Passiflora edulis f. flavicarpa).
Auteurs : Lydia K. Asande [Kenya] ; Richard O. Omwoyo [Kenya] ; Richard O. Oduor [Kenya] ; Evans N. Nyaboga [Kenya]Source :
- Plant methods [ 1746-4811 ] ; 2020.
Abstract
Background
Passion fruit (
Results
In the present study, we have developed a simple and fast
Conclusions
The developed transformation protocol is simple and rapid and could be useful for functional genomic studies and transferring agronomically important traits into passion fruit hybrid KPF4. This study developed a method that can be used to transfer traits such as resistance to viral diseases, low fruit quality and short storage life. To the best of our knowledge, this is the first report on genetic transformation system for commercial passion fruit hybrid KPF4.
DOI: 10.1186/s13007-020-00684-4
PubMed: 33088337
PubMed Central: PMC7565748
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A simple and fast <i>Agrobacterium</i>-mediated transformation system for passion fruit KPF4 (<i>Passiflora edulis</i> f. <i>edulis</i> × <i>Passiflora edulis</i> f. <i>flavicarpa</i>).</title><author><name sortKey="Asande, Lydia K" sort="Asande, Lydia K" uniqKey="Asande L" first="Lydia K" last="Asande">Lydia K. Asande</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Science, Kenyatta University, Nairobi, P.O. Box 43844 - 00100, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Plant Science, Kenyatta University, Nairobi, P.O. Box 43844 - 00100</wicri:regionArea><wicri:noRegion>P.O. Box 43844 - 00100</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of Nairobi, Nairobi, P.O. Box 30197 - 00100, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Biochemistry, University of Nairobi, Nairobi, P.O. Box 30197 - 00100</wicri:regionArea><wicri:noRegion>P.O. Box 30197 - 00100</wicri:noRegion></affiliation></author><author><name sortKey="Omwoyo, Richard O" sort="Omwoyo, Richard O" uniqKey="Omwoyo R" first="Richard O" last="Omwoyo">Richard O. Omwoyo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Science, Kenyatta University, Nairobi, P.O. Box 43844 - 00100, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Plant Science, Kenyatta University, Nairobi, P.O. Box 43844 - 00100</wicri:regionArea><wicri:noRegion>P.O. Box 43844 - 00100</wicri:noRegion></affiliation></author><author><name sortKey="Oduor, Richard O" sort="Oduor, Richard O" uniqKey="Oduor R" first="Richard O" last="Oduor">Richard O. Oduor</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Biotechnology, Kenyatta University, Nairobi, P.O. Box 43844 - 00100, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Biochemistry and Biotechnology, Kenyatta University, Nairobi, P.O. Box 43844 - 00100</wicri:regionArea><wicri:noRegion>P.O. Box 43844 - 00100</wicri:noRegion></affiliation></author><author><name sortKey="Nyaboga, Evans N" sort="Nyaboga, Evans N" uniqKey="Nyaboga E" first="Evans N" last="Nyaboga">Evans N. Nyaboga</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of Nairobi, Nairobi, P.O. Box 30197 - 00100, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Biochemistry, University of Nairobi, Nairobi, P.O. Box 30197 - 00100</wicri:regionArea><wicri:noRegion>P.O. Box 30197 - 00100</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:33088337</idno><idno type="pmid">33088337</idno><idno type="doi">10.1186/s13007-020-00684-4</idno><idno type="pmc">PMC7565748</idno><idno type="wicri:Area/Main/Corpus">000005</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000005</idno><idno type="wicri:Area/Main/Curation">000005</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000005</idno><idno type="wicri:Area/Main/Exploration">000005</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A simple and fast <i>Agrobacterium</i>-mediated transformation system for passion fruit KPF4 (<i>Passiflora edulis</i> f. <i>edulis</i> × <i>Passiflora edulis</i> f. <i>flavicarpa</i>).</title><author><name sortKey="Asande, Lydia K" sort="Asande, Lydia K" uniqKey="Asande L" first="Lydia K" last="Asande">Lydia K. Asande</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Science, Kenyatta University, Nairobi, P.O. Box 43844 - 00100, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Plant Science, Kenyatta University, Nairobi, P.O. Box 43844 - 00100</wicri:regionArea><wicri:noRegion>P.O. Box 43844 - 00100</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of Nairobi, Nairobi, P.O. Box 30197 - 00100, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Biochemistry, University of Nairobi, Nairobi, P.O. Box 30197 - 00100</wicri:regionArea><wicri:noRegion>P.O. Box 30197 - 00100</wicri:noRegion></affiliation></author><author><name sortKey="Omwoyo, Richard O" sort="Omwoyo, Richard O" uniqKey="Omwoyo R" first="Richard O" last="Omwoyo">Richard O. Omwoyo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Science, Kenyatta University, Nairobi, P.O. Box 43844 - 00100, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Plant Science, Kenyatta University, Nairobi, P.O. Box 43844 - 00100</wicri:regionArea><wicri:noRegion>P.O. Box 43844 - 00100</wicri:noRegion></affiliation></author><author><name sortKey="Oduor, Richard O" sort="Oduor, Richard O" uniqKey="Oduor R" first="Richard O" last="Oduor">Richard O. Oduor</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Biotechnology, Kenyatta University, Nairobi, P.O. Box 43844 - 00100, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Biochemistry and Biotechnology, Kenyatta University, Nairobi, P.O. Box 43844 - 00100</wicri:regionArea><wicri:noRegion>P.O. Box 43844 - 00100</wicri:noRegion></affiliation></author><author><name sortKey="Nyaboga, Evans N" sort="Nyaboga, Evans N" uniqKey="Nyaboga E" first="Evans N" last="Nyaboga">Evans N. Nyaboga</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of Nairobi, Nairobi, P.O. Box 30197 - 00100, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Biochemistry, University of Nairobi, Nairobi, P.O. Box 30197 - 00100</wicri:regionArea><wicri:noRegion>P.O. Box 30197 - 00100</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant methods</title><idno type="ISSN">1746-4811</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>Background</b></p><p>Passion fruit (</p></div><div type="abstract" xml:lang="en"><p><b>Results</b></p><p>In the present study, we have developed a simple and fast </p></div><div type="abstract" xml:lang="en"><p><b>Conclusions</b></p><p>The developed transformation protocol is simple and rapid and could be useful for functional genomic studies and transferring agronomically important traits into passion fruit hybrid KPF4. This study developed a method that can be used to transfer traits such as resistance to viral diseases, low fruit quality and short storage life. To the best of our knowledge, this is the first report on genetic transformation system for commercial passion fruit hybrid KPF4.</p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">33088337</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>23</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1746-4811</ISSN><JournalIssue CitedMedium="Print"><Volume>16</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Plant methods</Title><ISOAbbreviation>Plant Methods</ISOAbbreviation></Journal><ArticleTitle>A simple and fast <i>Agrobacterium</i>-mediated transformation system for passion fruit KPF4 (<i>Passiflora edulis</i> f. <i>edulis</i> × <i>Passiflora edulis</i> f. <i>flavicarpa</i>).</ArticleTitle><Pagination><MedlinePgn>141</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13007-020-00684-4</ELocationID><Abstract><AbstractText Label="Background" NlmCategory="UNASSIGNED">Passion fruit (<i>Passiflora edulis</i> Sims) is an important horticultural crop in the tropics and subtropics, where it has great commercial potential due to high demand for fresh edible fruits and processed juice as well as source of raw materials in cosmetic industries. Genetic engineering shows great potential in passion fruit improvement and can compensate for the limitations of conventional breeding. Despite the success achieved in genetic modification of few passion fruit varieties, transgenic passion fruit production is still difficult for farmer-preferred cultivars. Therefore, it is important to establish a simple and fast <i>Agrobacterium</i>-mediated cell transformation of commercial hybrid passion fruit KPF4 (<i>Passiflora edulis</i> f. <i>edulis</i> × <i>Passiflora edulis</i> f. <i>flavicarpa</i>).</AbstractText><AbstractText Label="Results" NlmCategory="UNASSIGNED">In the present study, we have developed a simple and fast <i>Agrobacterium</i>-mediated transformation system for hybrid passion fruit KPF4 using leaf disc explants. Factors affecting the rate of transient beta (β)-glucuronidase (<i>gus</i>A) expression and consequently transformation efficiency were optimized as follows: <i>Agrobacterium</i> cell density with an OD<sub>600</sub> of 0.5, 30 min infection time, 3 days of co-cultivation duration and the incorporation of 200 µM acetosyringone into <i>Agrobacterium</i> infection suspension medium. Using the optimized conditions, transgenic plants of KPF4 were produced within 2 months with an average transformation efficiency of 0.67%. The β-glucuronidase (GUS) histochemical staining confirmed the expression and integration of an intron-containing <i>gus</i>A gene into transformed leaf discs and transgenic plant lines of KPF4. The presence of <i>gus</i>A gene in the transgenic plants was confirmed by polymerase chain reaction (PCR). The results confirmed that the <i>gus</i>A gene was efficiently integrated into the passion fruit genome.</AbstractText><AbstractText Label="Conclusions" NlmCategory="UNASSIGNED">The developed transformation protocol is simple and rapid and could be useful for functional genomic studies and transferring agronomically important traits into passion fruit hybrid KPF4. This study developed a method that can be used to transfer traits such as resistance to viral diseases, low fruit quality and short storage life. To the best of our knowledge, this is the first report on genetic transformation system for commercial passion fruit hybrid KPF4.</AbstractText><CopyrightInformation>© The Author(s) 2020.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Asande</LastName><ForeName>Lydia K</ForeName><Initials>LK</Initials><AffiliationInfo><Affiliation>Department of Plant Science, Kenyatta University, Nairobi, P.O. Box 43844 - 00100, Kenya.</Affiliation><Identifier Source="GRID">grid.9762.a</Identifier><Identifier Source="ISNI">0000 0000 8732 4964</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biochemistry, University of Nairobi, Nairobi, P.O. Box 30197 - 00100, Kenya.</Affiliation><Identifier Source="GRID">grid.10604.33</Identifier><Identifier Source="ISNI">0000 0001 2019 0495</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Omwoyo</LastName><ForeName>Richard O</ForeName><Initials>RO</Initials><AffiliationInfo><Affiliation>Department of Plant Science, Kenyatta University, Nairobi, P.O. Box 43844 - 00100, Kenya.</Affiliation><Identifier Source="GRID">grid.9762.a</Identifier><Identifier Source="ISNI">0000 0000 8732 4964</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Oduor</LastName><ForeName>Richard O</ForeName><Initials>RO</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Biotechnology, Kenyatta University, Nairobi, P.O. Box 43844 - 00100, Kenya.</Affiliation><Identifier Source="GRID">grid.9762.a</Identifier><Identifier Source="ISNI">0000 0000 8732 4964</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nyaboga</LastName><ForeName>Evans N</ForeName><Initials>EN</Initials><Identifier Source="ORCID">0000-0002-9467-975X</Identifier><AffiliationInfo><Affiliation>Department of Biochemistry, University of Nairobi, Nairobi, P.O. Box 30197 - 00100, Kenya.</Affiliation><Identifier Source="GRID">grid.10604.33</Identifier><Identifier Source="ISNI">0000 0001 2019 0495</Identifier></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Methods</MedlineTA><NlmUniqueID>101245798</NlmUniqueID><ISSNLinking>1746-4811</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agrobacterium-mediated transformation</Keyword><Keyword MajorTopicYN="N">Passiflora edulis Sims</Keyword><Keyword MajorTopicYN="N">Transformation efficiency</Keyword><Keyword MajorTopicYN="N">Transient gusA expression</Keyword></KeywordList><CoiStatement>Competing interestsThe authors declare that they have no competing interests.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>05</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>10</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>22</Day><Hour>5</Hour><Minute>37</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>23</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33088337</ArticleId><ArticleId IdType="doi">10.1186/s13007-020-00684-4</ArticleId><ArticleId IdType="pii">684</ArticleId><ArticleId IdType="pmc">PMC7565748</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Sci Food Agric. 2013 Mar 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Asande</name></noRegion><name sortKey="Asande, Lydia K" sort="Asande, Lydia K" uniqKey="Asande L" first="Lydia K" last="Asande">Lydia K. Asande</name><name sortKey="Nyaboga, Evans N" sort="Nyaboga, Evans N" uniqKey="Nyaboga E" first="Evans N" last="Nyaboga">Evans N. Nyaboga</name><name sortKey="Oduor, Richard O" sort="Oduor, Richard O" uniqKey="Oduor R" first="Richard O" last="Oduor">Richard O. Oduor</name><name sortKey="Omwoyo, Richard O" sort="Omwoyo, Richard O" uniqKey="Omwoyo R" first="Richard O" last="Omwoyo">Richard O. Omwoyo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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